"Everything should be as simple as possible,
but not simpler."
Contents © 2004-2011 Massachusetts
Overview and Rationale
much can be learned by investigating the response to injury in man,
many of our current and future scientific and therapeutic advances in
critical care medicine will continue to depend upon studies using experimental
models designed to mimic the injury response in man. As a collaborative
group in the initial funding period, the Model Validation Core (MVC) investigators used several
well-defined experimental injury models as an approach to corroborate
findings from human studies and to further advance our understanding
of the host response to injury and inflammation.
Functions of the Model Validation Core
The MVC used experimental models of tissue injury, blood loss, and
acute inflammation to determine genomic and proteomic changes and characterize
these in a fashion comparable to the human studies (proteomic and genomic
The core examined specific patterns of expression
of a cluster of genes in circulating immune cells and correlated these
with the gene expression patterns in tissue-fixed immune cells and to
the systemic release of regulatory proteins.
The Model Validation Core substantially accomplished what has been described in terms of development of comparable murine model Standard Operating Procedures (SOPs) and have collected sufficient model data, samples, and tissues for further study by analytical methods as they are developed in our data interpretation groups (DIGs) during at least the first two years of the renewal Program and likely beyond.
The Program has not funded further model experiments and feels that we have ample model data, samples, and tissues gathered in Years 1 – 5 to evaluate. However, the Program continues to support model studies by our DIG investigators well beyond Year 5 in our Program. The members of the MVC remain enthusiastic participants in the analytical phase of our program and continue to represent the relevance of the murine models to our human investigations.